Incident Report

Subform I: General Information

1. Report Type.

New incident report

Incident Report Number: 2021-2459

2. Registrant Information.

Registrant Reference Number: VOP

Registrant Name (Full Legal Name no abbreviations): VETO-PHARMA SAS

Address: 12-14 Rue de la Croix Martre

City: Palaiseau

Country: France

Postal Code: 91120

3. Select the appropriate subform(s) for the incident.

Environment

4. Date registrant was first informed of the incident.

14-MAY-21

5. Location of incident.

Country: CANADA

Prov / State: ALBERTA

6. Date incident was first observed.

25-MAR-21

Product Description

7. a) Provide the active ingredient and, if available, the registration number and product name (include all tank mixes). If the product is not registered provide a submission number.

Active(s)

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PMRA Registration No. 29092      PMRA Submission No.       EPA Registration No.

Product Name: Apivar (TM) Strips

• Active Ingredient(s)
  • AMITRAZ
    • Guarantee/concentration 3.3 %

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PMRA Registration No.       PMRA Submission No.       EPA Registration No.

Product Name: Formic acid

• Active Ingredient(s)
  • FORMIC ACID
    • Unknown

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7. b) Type of formulation.

Application Information

8. Product was applied?

Yes

9. Application Rate.

3

Other Units: Strips per brood box

10. Site pesticide was applied to (select all that apply).

Site: Personal use / Usage personnel

11. Provide any additional information regarding application (how it was applied, amount applied, the size of the area treated etc).

The beekeeper treated 864 hives with Apivar for 8 weeks, starting March 4th 2021. 3 strips were placed in deep hives, and 4 to 5 in larger hives.

To be determined by Registrant

12. In your opinion, was the product used according to the label instructions?

Unknown

Subform IV: Environment (includes plants insects and wildlife)

1. Type of organism affected

Terr. Invrtbrt-Honey Bee/Inv.Ter-Abeille

2. Common name(s)

Honey bee

3. Scientific name(s)

Unknown

4. Number of organisms affected

Unknown

5. Description of site where incident was observed

Fresh water

Terrestrial

Salt Water

6. Check all symptoms that apply

Death

Impairment of health

7. Describe symptoms and outcome (died, recovered, etc.).

The beekeeper treated 864 hives with Apivar for 8 weeks, starting March 4th 2021. 3 strips were placed in deep hives, and 4 to 5 in larger hives (number of frames unknown). On March 25th, after 3 weeks of treatment, he observed high numbers of mites in some of his colonies (number unknown). Not all treated colonies seem to be affected equally. After about 3 weeks of treatment, the strips were replaced to make sure they were in the brood area. The quantity of brood in the hives is unknown.On April 17th, after 6 weeks of treatment, bees with deformed wings were observed in the colonies.The beekeeper noticed some mortality after the strips were put in. The date of this event is unknown, as well as the number of colonies affected. The beekeeper mentioned the necessity to supplement the colonies with protein patties during the treatment. After the treatment with Apivar, two treatments with formic acid were administered, with a 24hr mite drop count from 1,5 to 329. The beekeeper also did mite washes in around 10% of the hives of each hives, finding 1 to 20 mites per wash.

8. a) Was the incident a result of (select all that apply)

N/A

Unknown

8. b) i) How many times has the product been applied this year?

Unknown

8. b) ii) What was the date of the last application?

Unknown

9. Did it rain

9. a) During application?

No

9. b) Up to 3 days after application?

No

10. a) Was there a buffer zone?

No

10. b) What type?

10. c) What was the size of the buffer zone?

11. a) Were environmental samples collected and analysed?

No

To be determined by Registrant

12. Severity classification (if there is more than one possible classification, select the most severe)

Major

13. Please provide supplemental information here

The lack of efficacy was first observed at early stages of the treatment. The numbers of affected and dead hives are unknown.There was no monitoring of the infestation before and during treatment. Therefore, a high initial infestation cannot be excluded. The observation of mites after 21 days could correspond with the life cycle of bees (egg to emergence in 21 days), with the varroa in the brood becoming phoretic.Furthermore, other causes cannot be excluded, like starvation of some colonies (common in this region and time of the year), of underlying disease (DWV for example).